384-Well Multiplexed Luminex Cytokine Assays for Lead Optimization

An Evaluation of Commercial Fluorescent Bead-Based Luminex Cytokine Assays

The recent introduction of fluorescent bead-based technology, allowing the measurement of multiples analytes in a single 25-50 microl sample has revolutionized the study of cytokine responses. However, such multiplex approaches may compromise the ability of these assays to accurately measure actual cytokine levels. This study evaluates the performance of three commercially available multiplex c...

Comparison of 2 Luminex-based Multiplexed Protein Assays for Quantifying Microglia Activation and Inflammatory Proteins

Automated Workflows for Luminex xMAP Assays

Bead-based, multiplexing assays such as those provided by the Luminex xMAP technology, have proven to be highly useful for biomarker identification and quantification from cells, tissues and body fluids. Applications include biological research, drug discovery and clinical diagnostics. The original technology based on the use of polystyrene microspheres tends to have arduous, manual work flows ...

Luminex® Assays High-throughput Multiplex Bead Based Assays

Panomics has an aggressive program for the development of a broad range of assays for the Luminex platform and similar instruments based on the xMAP technology. Currently we have hundreds of RNA targets available in 3–30 plex assays using our using our QuantiGene Plex Reagent Systems, with new targets being added weekly. Our Procarta® Human, Mouse and Rat Assays can quantitatively measure cytok...

ELISPOT Assays in 384-Well Format: Up to 30 Data Points with One Million Cells

Comprehensive immune monitoring requires that frequencies of T cells, producing different cytokines, are measured to establish the magnitude of Th1, Th2, and Th17 components of cell-mediated immunity. Antigen titration provides additional information about the affinity of T cell response. In tumor immunity, it is also advisable to account for determinant spreading by testing multiple epitopes. ...